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DARC 抗体 (N-Term)

This anti-DARC antibody is a 山羊 多克隆 antibody detecting DARC in WB, ELISA, IHC 和 FACS. Suitable for 人.
产品编号 ABIN2465620
发货至: 中国

Quick Overview for DARC 抗体 (N-Term) (ABIN2465620)

抗原

See all DARC 抗体
DARC (Duffy Blood Group, Chemokine Receptor (DARC))

适用

  • 42
  • 11
  • 6

宿主

  • 31
  • 10
  • 2
  • 2
山羊

克隆类型

  • 33
  • 11
  • 1
多克隆

标记

  • 31
  • 4
  • 3
  • 3
  • 2
  • 2
This DARC antibody is un-conjugated

应用范围

  • 29
  • 23
  • 13
  • 8
  • 8
  • 6
  • 6
  • 5
  • 3
  • 2
  • 2
  • 1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)
  • 抗原表位

    • 9
    • 8
    • 6
    • 6
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    N-Term

    纯化方法

    Duffy antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Duffy antibody was raised against a 19 amino acid synthetic peptide near the N-Terminus of Duffy.
  • 应用备注

    ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 35 kDa band observed in human kidney and human liver lysates (calculated MW of 35.6 kDa according to NP_002027.2). Recommended concentration: 0.1-0.3 μg/mL. Immunohistochemistry: In paraffin embedded human kidney, stains epithelial cells of renal tubules and endothelial cells of vessels. The granular staining is compatible with the endoplasmic reticulum association of the protein. This antibody has also been successfully used on Huamn lung showing endothelial staining. Recommended concentration, 3 μg/mL. FACS: A customer has reported successful staining of HEK293S-TetR transfected with DARC receptor at a dilution of 1/1000.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    500 μg/mL

    缓冲液

    Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20 °C.
  • 抗原

    DARC (Duffy Blood Group, Chemokine Receptor (DARC))

    基因ID

    2532

    NCBI登录号

    NP_002027

    UniProt

    Q16570
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